papA

168

Xaa-Pro amino-peptidase

Locus: BSU_24460

Molecular weight: 37.96 kDa

Isoelectric point: 4.83

Protein length: 353 aa

Gene length: 1062 bp

Function: degradation of proline-containing peptides

Product: Xaa-Pro amino-peptidase

Essential: no

E.C.: 3.4.11.9

Synonyms: papA, yqhT

Genomic Context

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List of homologs in different organisms, belongs to COG0006 (Galperin et al., 2021)

Gene

Coordinates: 2,538,697  2,539,758

Phenotypes of a mutant

• inactivation of ''papA'' reduces sporulation efficiency to 1.2% that of wild type cells; delayed entry into sporulation PubMed

The protein

Protein family

• peptidase M24B family (with PapB, according to UniProt)

Structure

• 3Q6D (PDB) (the B. anthracis enzyme, 65% identity, 82% similarity)
• P54518 (AlphaFold)

Paralogous protein(s)

• PapB

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: figP-yqhS-papA-efp

  Description: PubMed

• Genes: yqhS-papA-efp

  Description: PubMed

Biological materials

Mutant

• MGNA-C447 (yqhT::erm), available at the NBRP B. subtilis, Japan
• available in Erhard Bremer's lab
• BKE24460 (papA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCATCTTGATTCTCCCCC,  downstream forward: _UP4_TGATTGGAATATAGGAGGAC
• BKK24460 (papA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCATCTTGATTCTCCCCC,  downstream forward: _UP4_TGATTGGAATATAGGAGGAC

Expression vectors

• pGP769: expression of Strep-papA by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
• pGP1150: expression of papA-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Labs working on this gene/protein

• Erhard Bremer, Marburg University, Germany Homepage

References

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