bdr

168

thioredoxin reductase-like flavoprotein with bacillithiol-disulfide (BSSB) reductase activity, acts also as bacilliredoxin reductase

Locus: BSU_22950

Molecular weight: 36.18 kDa

Isoelectric point: 6.08

Protein length: 324 aa

Gene length: 975 bp

Function: recycling of oxidized bacillithiol disulfide to the reduced form (BSH)

Product: FAD-containing NADPH-dependent bacillithiol disulfide reductase

Essential: no

Synonyms: ypdA

Genomic Context

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List of homologs in different organisms, belongs to COG0492 (Galperin et al., 2021)

Gene

Coordinates: 2,400,984 → 2,401,958

The protein

Catalyzed reaction/ biological activity

• recycling of oxidized bacillithiol disulfide (BSSB) to the reduced form (BSH) PubMed
• bacilliredoxin reductase activity PubMed

Cofactors

• FAD
• NADPH

Structure

• 4ZN0 (PDB) (from Methanosarcina mazei, 27% identity) PubMed
• P50736 (AlphaFold)

Localization

• cell membrane (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-A398 (ypdA::erm), available at the NBRP B. subtilis, Japan
• BKE22950 (Δbdr::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTGTCAACTCCTGCG,  downstream forward: _UP4_TAAAGATGAGGAGCATATGA
• BKK22950 (Δbdr::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTGTCAACTCCTGCG,  downstream forward: _UP4_TAAAGATGAGGAGCATATGA

References

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