ArgF

• Description: ornithine carbamoyltransferase
  
  

Gene name	argF
Synonyms
Essential	no
Product	ornithine carbamoyltransferase
Function	biosynthesis of arginine
Gene expression levels in SubtiExpress: argF
Metabolic function and regulation of this protein in SubtiPathways: argF
MW, pI	34 kDa, 4.965
Gene length, protein length	957 bp, 319 aa
Immediate neighbours	carB, yjzC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

• Locus tag: BSU11250

Database entries

• BsubCyc: BSU11250

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline (according to Swiss-Prot)

• Protein family: ATCase/OTCase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU11250

• Structure: 2EF0 (from Thermus thermophilus hb8, 48% identity, 63% similarity)

• UniProt: P18186

• KEGG entry: [3]

• E.C. number: 2.1.3.3

Expression and regulation

• Operon: argC-argJ-argB-argD-carA-carB-argF PubMed

• Expression browser: argF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by arginine (AhrC) PubMed

• Regulatory mechanism:
  • AhrC: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1084 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1644 PubMed

Biological materials

• Mutant:
  • 1A605 ( argF::erm), PubMed, available at BGSC
  • 1A606 ( argF::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)