ydjO

168

unknown

Locus: BSU_06270

Molecular weight: 7.71 kDa

Isoelectric point: 7.64

Protein length: 69 aa

Gene length: 210 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ydjO

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 681,255  681,464

The protein

Structure

• O34759 (AlphaFold)

Modification

• phosphorylated on Arg-7 PubMed

Expression and Regulation

Operons

• Genes: yeaA-ydjP-ydjO

  Description: PubMed

  Regulation

  • induced by cold shock PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon
  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-C222 (ydjO::erm), available at the NBRP B. subtilis, Japan
• BKE06270 (ydjO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAGTCACTTCTTCCCT,  downstream forward: _UP4_TAGTTCTTCCCTATAACAAA
• BKK06270 (ydjO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAGTCACTTCTTCCCT,  downstream forward: _UP4_TAGTTCTTCCCTATAACAAA

Expression vectors

• pGP3563 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab

References

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