yvoE

168

putative glycerol-3-phosphatase (has been mis-annotated as HPr-P phosphatase)

Locus: BSU_34970

Molecular weight: 23.86 kDa

Isoelectric point: 4.49

Protein length: 216 aa

Gene length: 651 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yvoE, hprP

Genomic Context

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List of homologs in different organisms, belongs to COG0546 (Galperin et al., 2021)

Gene

Coordinates: 3,591,810 → 3,592,460

The protein

Catalyzed reaction/ biological activity

• glycerol-3-phosphate -→ glycerol + inorganic phosphate PubMed
• diphosphate + H₂O --> H⁺ + 2 phosphate (according to UniProt)

Protein family

• HAD superfamily (according to UniProt)

Structure

• 2YY6 (PDB) (from Aquifex Aeolicus 36% identity)
• Q9JMQ2 (AlphaFold)

Expression and Regulation

Operons

• Genes: hprK-lgt-yvoD-yvoE-yvoF

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A390 (yvoE::erm), available at the NBRP B. subtilis, Japan
• GP852 (ΔyvoE::aphA3), available in Jörg Stülke's lab
• GP851 (Δ(lgt-yvoD-yvoE-yvoF)::aphA3), available in Jörg Stülke's lab
• BKE34970 (ΔyvoE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCGTCGTTACTTGTTTGT,  downstream forward: _UP4_CAAATCGTTGGAGTGAAGTA
• BKK34970 (ΔyvoE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCGTCGTTACTTGTTTGT,  downstream forward: _UP4_CAAATCGTTGGAGTGAAGTA

References

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