yxeB

168

hydroxamate siderophore ABC transporter (only ferrioxamine) (binding protein)

Locus: BSU_39610

Molecular weight: 35.10 kDa

Isoelectric point: 5.53

Protein length: 321 aa

Gene length: 966 bp

Function: siderophore uptake

Product: hydroxamate siderophore ABC transporter

Essential: no

Synonyms: yxeB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)

Gene

Coordinates: 4,067,183  4,068,148

Phenotypes of a mutant

• no growth with the xenosiderophore ferrioxamine E as single source of iron PubMed

The protein

Protein family

• Bacterial solute-binding protein 8 family (according to UniProt)

Domains

• Fe/B12 periplasmic-binding domain (aa 58-316) (according to UniProt)

Structure

• 4FNA (PDB) (FhuD from Staphylococcus aureus, 35% identity) PubMed
• P54941 (AlphaFold)

Paralogous protein(s)

• FhuD

Localization

• extracellular (signal peptide) PubMed
• membrane, lipid modification as retention signal PubMed

Expression and Regulation

Operons

• Genes: yxeB

  Description: PubMed

  Regulation

  • repressed unless the cells enter an iron starvation (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

Biological materials

Mutant

• MGNA-B706 (yxeB::erm), available at the NBRP B. subtilis, Japan
• BKE39610 (yxeB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAGAACCCTCCCACTA,  downstream forward: _UP4_TAAAAAAACGGCACAGTCAT
• BKK39610 (yxeB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAGAACCCTCCCACTA,  downstream forward: _UP4_TAAAAAAACGGCACAGTCAT

References

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