purE

purE
168

phosphoribosylaminoimidazole carboxylase (ATP-dependent)

Locus
BSU_06420
Molecular weight
17.03 kDa
Isoelectric point
5.2
Protein length
Gene length
Function
purine biosynthesis
Product
phosphoribosylaminoimidazole carboxylase (ATP-dependent)
Essential
no
E.C.
4.1.1.21
Synonyms
purE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0041 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
698,612  699,100
The protein
Catalyzed reaction/ biological activity
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H+ --> 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate (according to UniProt)
Protein family
AIR carboxylase family ( single member, according to UniProt)
Structure
1O4V (PDB) (from Thermotoga maritima, 53% identity, 64% similarity) PubMed
Expression and Regulation
Operons
Description
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purEpurD

2025-07-16 17:01:58

Jstuelk

259

ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06420 (purE::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTCTGTTCCCCACCTTCT,  downstream forward: _UP4_ACAGTGTTAGAAAGCAGTGA
BKK06420 (purE::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTCTGTTCCCCACCTTCT,  downstream forward: _UP4_ACAGTGTTAGAAAGCAGTGA
References
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Time of last update: 2025-07-17 22:14:45

Author of last update: Jstuelk