ddl

168

D-alanine-D-alanine ligase

Locus: BSU_04560

Molecular weight: 38.97 kDa

Isoelectric point: 4.58

Protein length: 354 aa

Gene length: 1065 bp

Function: peptidoglycan precursor biosynthesis

Product: D-alanine-D-alanine ligase

Essential: yes

E.C.: 6.3.2.4

Synonyms: ddl, ddlA

Genomic Context

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List of homologs in different organisms, belongs to COG1181 (Galperin et al., 2021)

Gene

Coordinates: 508,248  509,312

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + 2 D-alanine --> ADP + phosphate + D-alanyl-D-alanine (according to UniProt)

Protein family

• D-alanine--D-alanine ligase family (single member, according to UniProt)

Domains

• ATP-grasp doamin (aa 133-338) (according to UniProt)

Structure

• 2YZG (PDB) (from Thermus thermophilus hb8 mutant, 40% identity, 53% similarity)
• P96612 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ddl-murF

  Description: PubMed

  Regulation

  • expression is reduced in a SigV mutant PubMed
  • the leader mRNA is processed upstream of ddl by RNase Y, this requires the RicA-RicF-RicT complex PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in sigM regulon
  • SigV: sigma factor, in sigV regulon

Biological materials

Mutant

• MGNA-C104 (ddlA::erm), available at the NBRP B. subtilis, Japan

References

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