AroA

• Description: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase / chorismate mutase-isozyme 3
  
  

Gene name	aroA
Synonyms	aroG
Essential	no
Product	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase / chorismate mutase-isozyme 3
Function	biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroA
Metabolic function and regulation of this protein in SubtiPathways: aroA
MW, pI	39 kDa, 5.341
Gene length, protein length	1074 bp, 358 aa
Immediate neighbours	ccpA, ytxJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins, most abundant proteins

The gene

Basic information

• Locus tag: BSU29750

Database entries

• BsubCyc: BSU29750

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Phosphoenolpyruvate + D-erythrose 4-phosphate + H₂O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate (according to Swiss-Prot)

• Protein family: class-I DAHP synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylation on Ser-2 PubMed
  • phosphorylation on Thr-4 PubMed
  • phosphorylated on Arg-45 and Arg-301 PubMed
  • Cys126 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed

• Cofactors:

• Effectors of protein activity: subject to feedback inhibition PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU29750

• Structure: 1VR6 (from Thermotoga maritima, 51% identity, 68% similarity)

• UniProt: P39912

• KEGG entry: [3]

• E.C. number: 2.5.1.54 5 and 5.4.99.5

Expression and regulation

• Operon: aroA PubMed

• Expression browser: aroA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • subject to feedback inhibition PubMed
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 20630 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 13334 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: