aroB

168

3-dehydroquinate synthase

Locus: BSU_22700

Molecular weight: 40.66 kDa

Isoelectric point: 6.94

Protein length: 362 aa

Gene length: 1089 bp

Function: biosynthesis of aromatic amino acids

Product: 3-dehydroquinate synthase

Essential: no

E.C.: 4.2.3.4

Synonyms: aroB

Genomic Context

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List of homologs in different organisms, belongs to COG0337 (Galperin et al., 2021)

Gene

Coordinates: 2,378,012  2,379,100

The protein

Catalyzed reaction/ biological activity

• 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate --> 3-dehydroquinate + phosphate (according to UniProt)

Protein family

• sugar phosphate cyclases superfamily (single member, according to UniProt)

Structure

• 3CLH (PDB) (from Helicobacter pylori, 36% identity) PubMed
• P31102 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: cheR-aroF-aroB-aroH

  Description: PubMed

• Genes: aroF-aroB-aroH

  Description: PubMed

Biological materials

Mutant

• 1A613 ( aroB::erm), PubMed, available at BGSC
• BKE22700 (aroB::erm  trpC2) available at BGSC and in Jörg Stülke's lab,  PubMed, upstream reverse: _UP1_AGTTTGAACATGCAGTGTCT,  downstream forward: _UP4_AAATGGCGATTGGAGGAGAC
• BKK22700 (aroB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGTTTGAACATGCAGTGTCT,  downstream forward: _UP4_AAATGGCGATTGGAGGAGAC

References

Reviews

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Original Publications

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