comFC

168

late competence gene, resembles type 1 phosphoribosyl transferases

Locus: BSU_35450

Molecular weight: 26.26 kDa

Isoelectric point: 8.85

Protein length: 229 aa

Gene length: 690 bp

Function: nucleotide scavenging

Product: unknown

Essential: no

Synonyms: comFC

Genomic Context

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Categories containing this gene/protein

Information processing, Genetics, Genetic competence

Lifestyles, Exponential and early post-exponential lifestyles, Genetic competence

Groups of genes, Phosphoproteins, Phosphorylation on an Arg residue

List of homologs in different organisms, belongs to COG1040 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, ComK regulon, DegU regulon, ScoC regulon

Gene

Coordinates: 3,641,125  3,641,814

The protein

Protein family

comF/gntX family (single member, according to UniProt)

Structure

P39147 (AlphaFold)

Modification

phosphorylated on Arg-74 PubMed

Localization

localizes to one or both cell poles PubMed

Expression and Regulation

Operons

Genes: comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL-yviE-fliW-csrA

Description: PubMed

Expression

expression is heterogeneous PubMed

Regulatory mechanism

ComK: activation, PubMed, in comK regulon
DegU: activation, DegU-P, PubMed, in degU regulon
ScoC: repression, PubMed, in scoC regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

GP4447 (trpC2 ΔcomFC::neo), available in Jörg Stülke's lab
BKE35450 (comFC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCGAATCACATAATAAAC,  downstream forward: _UP4_TAAATGATTCTGTTTTTATG
BKK35450 (comFC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCGAATCACATAATAAAC,  downstream forward: _UP4_TAAATGATTCTGTTTTTATG

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

References

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