aapA

168

amino acid permease

Locus: BSU_27090

Molecular weight: 50.49 kDa

Isoelectric point: 9.68

Protein length: 462 aa

Gene length: 1389 bp

Function: amino acid uptake

Product: amino acid permease

Essential: no

Synonyms: aapA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1113 (Galperin et al., 2021)

Gene

Coordinates: 2,766,558 → 2,767,946

The protein

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

Structure

• 7CCS (PDB) (human protein, corresponds to aa 6 ... 420, 25% identity) PubMed
• O06005 (AlphaFold)

Paralogous protein(s)

• GabP, AlaP, HutM, YvbW, AimB, RocC, RocE, YbgF, YdgF

Expression and Regulation

Operons

• Genes: aapA

  Description: PubMed

Biological materials

Mutant

• GP2377 Δ''aapA::tet'', available in Jörg Stülke's lab PubMed
• MGNA-A230 (aapA::erm), available at the NBRP B. subtilis, Japan
• BKE27090 (ΔaapA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTTATTTTCCTCCAGT,  downstream forward: _UP4_TAAAAAAAAGACAAGGGATA
• BKK27090 (ΔaapA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTTATTTTCCTCCAGT,  downstream forward: _UP4_TAAAAAAAAGACAAGGGATA

LacZ fusion

• pGP2273 (in pAC5) (GP2960), available in Jörg Stülke's lab

References

Research papers

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