yvmC

168

cyclodipeptide synthase

Locus: BSU_35070

Molecular weight: 28.36 kDa

Isoelectric point: 6.61

Protein length: 248 aa

Gene length: 747 bp

Function: biosynthesis of the extracellular iron chelator pulcherrimin

Product: cyclodipeptide synthase

Essential: no

Synonyms: yvmC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms

Gene

Coordinates: 3,603,821 → 3,604,567

The protein

Catalyzed reaction/ biological activity

• synthesis of the cyclic dipeptide cyclo-L-leucyl-L-leucyl with the corresponding charged tRNAs as substrates in an ATP-dependent manner PubMed
• 2 L-leucyl-tRNA^Leu --> cyclo(L-leucyl-L-leucyl) + 2 H⁺ + 2 tRNA^Leu (according to UniProt)

Protein family

• CDPS family (single member, according to UniProt)

Structure

• 3S7T (PDB) (from B. licheniformis, 70% identity, 86% similarity) PubMed
• O34351 (AlphaFold)

Expression and Regulation

Operons

• Genes: yvmC-cypX

  Description: Reference

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • PchR: repression, PubMed, in pchR regulon
  • ScoC: repression, PubMed, in scoC regulon

Biological materials

Mutant

• BKE35070 (ΔyvmC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCATTCACCCCTAAAA,  downstream forward: _UP4_TGATAGGGGGAGTAAAACAT
• BKK35070 (ΔyvmC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCATTCACCCCTAAAA,  downstream forward: _UP4_TGATAGGGGGAGTAAAACAT

References

Loading