cypX

168

cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase

Locus: BSU_35060

Molecular weight: 45.31 kDa

Isoelectric point: 5.31

Protein length: 405 aa

Gene length: 1218 bp

Function: biosynthesis of the extracellular iron chelator pulcherrimin

Product: cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase

Essential: no

Synonyms: cypX

Genomic Context

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List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

Gene

Coordinates: 3,602,588 → 3,603,805

The protein

Catalyzed reaction/ biological activity

• implicated in the three-step oxidative transformation of the diketopiperazine cyclo-l-leucyl-l-leucyl into pulcherriminic acid PubMed
• cyclo(L-leucyl-L-leucyl) + 4 H⁺ + 3 O₂ + 6 reduced [2Fe-2S]-[ferredoxin] --> 4 H₂O + 6 oxidized [2Fe-2S]-[ferredoxin] + pulcherriminic acid (according to UniProt)

Protein family

• cytochrome P450 family (according to UniProt)

Structure

• 3NC3 (PDB) PubMed
• O34926 (AlphaFold)

Expression and Regulation

Operons

• Genes: yvmC-cypX

  Description: Reference

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • PchR: repression, PubMed, in pchR regulon
  • ScoC: repression, PubMed, in scoC regulon

Biological materials

Mutant

• MGNA-A333 (cypX::erm), available at the NBRP B. subtilis, Japan
• BKE35060 (ΔcypX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGGCTCATGTTTTACTCCC,  downstream forward: _UP4_TAATAGAATTCCAAAGGTCT
• BKK35060 (ΔcypX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGGCTCATGTTTTACTCCC,  downstream forward: _UP4_TAATAGAATTCCAAAGGTCT

References

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