ctaB

168

heme O synthase (major enzyme)

locus: BSU_14880

Molecular weight: 33.79 kDa

pI: 9.57

Protein length: 305 aa

Gene length: 918 bp

function: heme biosynthesis

product: heme O synthase (major enzyme)

essential: no

ec: 2.5.1.-

synonyms: ctaB

List of homologs in different organisms, belongs to COG0109 (Galperin et al., 2021)

Gene

Coordinates: 1,559,309  1,560,226

Phenotypes of a mutant

• inactivation of ''ctaB'' facilitates without a wall (due to reduction of oxidative stress) [Pubmed|26051891]

The protein

Catalyzed reaction/ biological activity

• (2E,6E)-farnesyl diphosphate + H₂O + heme b --> diphosphate + Fe(II)-heme o (according to UniProt)

Protein family

• UbiA prenyltransferase family (with [protein|6CE139563BBD7C1C97AEC3AA3F88146C4EEA79E4|ctaO], according to UniProt)

Structure

• [AF|P24009]

Paralogous protein(s)

• [protein|6CE139563BBD7C1C97AEC3AA3F88146C4EEA79E4|ctaO]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|83F5D5FCCAFADB7231ACD9BE7941820240954C70|ctaB]-[gene|72D5442C0EF3B404F7B615366E357B68C863CEB6|ctaC]-[gene|20D1174554A03EECE233A1A045F6DF83AF799959|ctaD]-[gene|9DF8423F1D63ED3928B2818F58BC3DECCDBB1DDB|ctaE]-[gene|28E5AF86CE49D7B0ECBFAF9ED5C1FC65D9F810EA|ctaF]-[gene|8C16E115CB59A74C24954BFCDE112720BA09D5EA|ctaG]

  description: [Pubmed|9829923]

  regulation

  • expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]) [Pubmed|9829923]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|9829923], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: repression, synergistic repression with [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB], in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, synergistic repression with [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

Biological materials

Mutant

• BKE14880 ([gene|83F5D5FCCAFADB7231ACD9BE7941820240954C70|ctaB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACGTAAACCTCCCTTA,  downstream forward: _UP4_TAAAAAATAGATATGATGCT
• BKK14880 ([gene|83F5D5FCCAFADB7231ACD9BE7941820240954C70|ctaB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACGTAAACCTCCCTTA,  downstream forward: _UP4_TAAAAAATAGATATGATGCT

Reviews

• 35630371

References

• 7968515,15491161,9829923,19204012,26051891