ctaB

168

heme O synthase (major enzyme)

Locus: BSU_14880

Molecular weight: 33.79 kDa

Isoelectric point: 9.57

Protein length: 305 aa

Gene length: 918 bp

Function: heme biosynthesis

Product: heme O synthase (major enzyme)

Essential: no

E.C.: 2.5.1.-

Synonyms: ctaB

Genomic Context

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List of homologs in different organisms, belongs to COG0109 (Galperin et al., 2021)

Gene

Coordinates: 1,559,309  1,560,226

Phenotypes of a mutant

• inactivation of ctaB facilitates without a wall (due to reduction of oxidative stress) PubMed

The protein

Catalyzed reaction/ biological activity

• (2E,6E)-farnesyl diphosphate + H₂O + heme b --> diphosphate + Fe(II)-heme o (according to UniProt)

Protein family

• UbiA prenyltransferase family (with CtaO, according to UniProt)

Structure

• P24009 (AlphaFold)

Paralogous protein(s)

• CtaO

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ctaB-ctaC-ctaD-ctaE-ctaF-ctaG

  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in resD regulon
  • Abh: repression, synergistic repression with AbrB, in abh regulon
  • CcpA: repression, in ccpA regulon
  • AbrB: repression, synergistic repression with Abh, in abrB regulon

Biological materials

Mutant

• BKE14880 (ctaB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACGTAAACCTCCCTTA,  downstream forward: _UP4_TAAAAAATAGATATGATGCT
• BKK14880 (ctaB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACGTAAACCTCCCTTA,  downstream forward: _UP4_TAAAAAATAGATATGATGCT

Reviews

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References

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