comGB

168

polytopic membrane protein, DNA transport machinery

Locus: BSU_24720

Molecular weight: 36.90 kDa

Isoelectric point: 8.65

Protein length: 323 aa

Gene length: 972 bp

Function: genetic competence, DNA uptake

Product: DNA transport machinery

Essential: no

Synonyms: comGB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1459 (Galperin et al., 2021)

Gene

Coordinates: 2,557,983 → 2,558,954

The protein

Protein family

• GSP F family (single member, according to UniProt)

Structure

• P25954 (AlphaFold)

Localization

• cell membrane, polar localization PubMed

Expression and Regulation

Operons

• Genes: comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL

  Description: PubMed

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigF: sigma factor, PubMed, in sigF regulon

  Additional information

  • the ''comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL'' operon requires NusA for expression Reference

Biological materials

Mutant

• BKE24720 (ΔcomGB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCACCGAGCCTCTTTA,  downstream forward: _UP4_AATCAAATGTGAAAGGAAGA
• BKK24720 (ΔcomGB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCACCGAGCCTCTTTA,  downstream forward: _UP4_AATCAAATGTGAAAGGAAGA
• BP914 (Δ(comGA-comGB-comGC-comGD-comGE-comGF-comGG)::phleo), available in Fabian Commichau's lab

References

Reviews

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Original Publications

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