tepA

168

orphan [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-like [wiki|germination] protease, contributes to SASP degradation

locus: BSU_16790

Molecular weight: 24.10 kDa

pI: 5.57

Protein length: 245 aa

Gene length: 738 bp

function: degradation of SASPs

product: [wiki|germination] protease

essential: no

synonyms: tepA, ymfB, ylxI

List of homologs in different organisms, belongs to COG0740 (Galperin et al., 2021)

Gene

Coordinates: 1,751,201  1,751,938

The protein

Catalyzed reaction/ biological activity

• degradation of SASPs in germinating spores [Pubmed|23927687]

Protein family

• Peptidase S14 family (with [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP], according to UniProt)

Structure

• [AF|Q99171]

Effectors of protein activity

• activity requires [protein|A7C95C19ABD47456351C05A88B14F932BDEF1059|ylzJ] [Pubmed|23927687]

[wiki|Localization]

• inner spore membrane [Pubmed|26731423]

Expression and Regulation

Operons

• genes: [gene|A16C6515D185E3A268D57D77F28EF7EF9AF9FE05|tepA]-[gene|A7C95C19ABD47456351C05A88B14F932BDEF1059|ylzJ]

  description: [Pubmed|22383849]

  regulation

  • expressed late during [wiki|sporulation] in the forespore ([protein|search|SigG], [wiki|SpoVT]) [Pubmed|23123912]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|23123912], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|23123912], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-B377 (ymfB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1376 NBRP B. subtilis, Japan]
• GP1120 (spc), available in [wiki|Jörg Stülke]'s lab
• BKE16790 ([gene|A16C6515D185E3A268D57D77F28EF7EF9AF9FE05|tepA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCGCTTTCATCCTTTC,  downstream forward: _UP4_AAAGAAGAAGGACGGATGAT
• BKK16790 ([gene|A16C6515D185E3A268D57D77F28EF7EF9AF9FE05|tepA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCGCTTTCATCCTTTC,  downstream forward: _UP4_AAAGAAGAAGGACGGATGAT

References

Reviews

• 35638784

Original Publications

• 23123912,22383849,10455123,23927687,26731423