tepA

168

orphan ClpP-like germination protease, contributes to SASP degradation, required for efficient translocation of pre-proteins across the membrane

Locus: BSU_16790

Molecular weight: 24.10 kDa

Isoelectric point: 5.57

Protein length: 245 aa

Gene length: 738 bp

Function: protein

Product: translocation-enhancing protein TepA

Essential: no

Synonyms: tepA, ymfB, ylxI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0740 (Galperin et al., 2021)

Gene

Coordinates: 1,751,201  1,751,938

The protein

Catalyzed reaction/ biological activity

• degradation of SASPs in germinating spores PubMed

Protein family

• Peptidase S14 family (with ClpP, according to UniProt)

Structure

• Q99171 (AlphaFold)

Effectors of protein activity

• activity requires YlzJ PubMed

Localization

• inner spore membrane PubMed

Expression and Regulation

Operons

• Genes: tepA-ylzJ

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

  Regulatory mechanism

  • SpoVT: repression, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-B377 (ymfB::erm), available at the NBRP B. subtilis, Japan
• GP1120 (spc), available in Jörg Stülke's lab
• BKE16790 (tepA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCGCTTTCATCCTTTC,  downstream forward: _UP4_AAAGAAGAAGGACGGATGAT
• BKK16790 (tepA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCGCTTTCATCCTTTC,  downstream forward: _UP4_AAAGAAGAAGGACGGATGAT

References

Reviews

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Original Publications

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