dgcK

168

diguanylate cyclase

Locus: BSU_09120

Molecular weight: 40.53 kDa

Isoelectric point: 8.57

Protein length: 359 aa

Gene length: 1080 bp

Function: synthesis of c-di-GMP

Product: diguanylate cyclase

Essential: no

Synonyms: dgcK, yhcK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2199 (Galperin et al., 2021)

Gene

Coordinates: 985,734 → 986,813

The protein

Catalyzed reaction/ biological activity

• synthesis of c-di-GMP from two molecules of GTP PubMed

Domains

• six transmembrane helices at the N-terminus (according to UniProt?)
• contains a C-terminal GGDEF domain (aa 223-357) PubMed

Structure

• 2WB4 (PDB) (the C-terminal GGDEF domain, PleD from Caulobacter vibrioides, 44% identity)
• P54595 (AlphaFold)

Localization

• cell membrane at cell poles and septa, probably close to YdaK PubMed
• present at a single site in the cell membrane PubMed

Expression and Regulation

Operons

• Genes: dgcK

  Description: PubMed

Biological materials

Mutant

• MGNA-A656 (yhcK::erm), available at the NBRP B. subtilis, Japan
• GP848 (''dgcK::ermC''), available in Jörg Stülke's lab PubMed
• BKE09120 (ΔdgcK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATATTATCACCCTTATA,  downstream forward: _UP4_TGAATTCAATGTTCGAATTC
• BKK09120 (ΔdgcK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATATTATCACCCTTATA,  downstream forward: _UP4_TGAATTCAATGTTCGAATTC

References

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