ydeA

168

glyoxalase III-like enzyme

Locus: BSU_05110

Molecular weight: 22.31 kDa

Isoelectric point: 5.64

Protein length: 197 aa

Gene length: 594 bp

Function: detoxification of methylglyoxal

Product: glyoxalase III-like enzyme

Essential: no

E.C.: null

Synonyms: ydeA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0693 (Galperin et al., 2021)

Gene

Coordinates: 558,408  559,001

The protein

Catalyzed reaction/ biological activity

• methylglyoxal - D-lactate PubMed

Protein family

• peptidase C56 family (according to UniProt)

Domains

• PfpI endopeptidase domain (aa 29-166) (according to UniProt)

Structure

• 3F5D (PDB)
• P96658 (AlphaFold)

Biological materials

Mutant

• MGNA-C128 (ydeA::erm), available at the NBRP B. subtilis, Japan
• BKE05110 (ydeA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAACTATATACCGCCTTA,  downstream forward: _UP4_TAATTTGCTGGAAATGATTT
• BKK05110 (ydeA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAACTATATACCGCCTTA,  downstream forward: _UP4_TAATTTGCTGGAAATGATTT

References

Loading