BsrG

• Description: hydrophobic toxin
  
  

Gene name	bsrG
Synonyms
Essential	no
Product	toxin
Function	unknown
MW, pI
Gene length, protein length	294 bp, 38 aa
Immediate neighbours	yolA, SR4
Get the DNA and protein
Genetic context File:BsrG context.gif This image was kindly provided by SubtiList

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, SPß prophage, membrane proteins

The gene

Basic information

• Locus tag:

Database entries

• BsubCyc: ?

• DBTBS entry: no entry

• SubtiList entry: no entry

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane protein (based on the presence of a trans-membrane domain) PubMed

Database entries

• BsubCyc: ?

• Structure:

• UniProt:

• KEGG entry:

• E.C. number:

Expression and regulation

• Operon: bsrG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression increases at the onset of the stationary phase PubMed
  • the mRNA is destabilized upon interaction with the antitoxin RNA SR4 PubMed
  • the mRNA is degraded by Rnc, Rny and Rnr PubMed

• Regulatory mechanism:
  • the mRNA is degraded by Rnc, Rny and Rnr, degradation is enhanced at higher temperature PubMed

• Additional information:
  • half-life of the mRNA: 15 min PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

Labs working on this gene/protein

Sabine Brantl, Jena, Germany

References

Natalie Jahn, Sabine Brantl
One antitoxin--two functions: SR4 controls toxin mRNA decay and translation.
Nucleic Acids Res: 2013, 41(21);9870-80
[PubMed:23969414] [WorldCat.org] [DOI] (I p)

Natalie Jahn, Heike Preis, Christoph Wiedemann, Sabine Brantl
BsrG/SR4 from Bacillus subtilis--the first temperature-dependent type I toxin-antitoxin system.
Mol Microbiol: 2012, 83(3);579-98
[PubMed:22229825] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shinichi Saito, Hiroshi Kakeshita, Kouji Nakamura
Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis.
Gene: 2009, 428(1-2);2-8
[PubMed:18948176] [WorldCat.org] [DOI] (P p)