yyaM

168

unknown

Locus: BSU_40810

Molecular weight: 32.74 kDa

Isoelectric point: 10.44

Protein length: 305 aa

Gene length: 918 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: yyaM

Genomic Context

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Categories containing this gene/protein

Groups of genes, Membrane proteins

Groups of genes, Proteins of unknown function

List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 4,190,209 → 4,191,126

The protein

Protein family

EamA transporter family (according to UniProt)

Domains

2 EamA domains (aa 15-140, aa 164-290) (according to UniProt)

Structure

5I20 (PDB) (from Ancylobacter novellus DSM 506, 25% identity) PubMed
P37511 (AlphaFold)

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes: yyaN-yyaM-yyaL

Description: PubMed

Biological materials

Mutant

MGNA-B861 (yyaM::erm), available at the NBRP B. subtilis, Japan
BKE40810 (ΔyyaM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATGTATATATTTAAAT,  downstream forward: _UP4_TAAAGAAATAGGGAGTATTT
BKK40810 (ΔyyaM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATGTATATATTTAAAT,  downstream forward: _UP4_TAAAGAAATAGGGAGTATTT

Expression system

GP4398 (trpC2 xkdE::P_xyl-yyaM-ermC), available in Jörg Stülke's lab

References

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