walH

168

negative effector of WalK, controls cell wall metabolism

Locus: BSU_40390

Molecular weight: 52.05 kDa

Isoelectric point: 5.59

Protein length: 455 aa

Gene length: 1368 bp

Function: control of cell wall metabolism

Product: negative effector of WalK

Essential: no

Synonyms: walH, yycH

Genomic Context

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List of homologs in different organisms, belongs to COG4863 (Galperin et al., 2021)

Gene

Coordinates: 4,150,496  4,151,863

Phenotypes of a mutant

• growth defect at high temperature, lysis at 49C, this can be suppressed by mutations that reduce WalK autokinase activity, by inactivation of lytE, or by overexpreesion of IseA or PdaC PubMed
• defective in Swarming motility (periodic cessation and reinitiation of Swarming motility, terracing colonies) PubMed

Additional information

• the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots PubMed

The protein

Structure

• 2FGT (PDB)
• Q794W0 (AlphaFold)

Localization

• cell membrane (according to UniProt), spotty close to the membrane PubMed

Expression and Regulation

Operons

• Genes: walR-walK-walH-walI-walJ-htrC

  Description: PubMed

  Regulation

  • htrC transcript expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-B826 (yycH::erm), available at the NBRP B. subtilis, Japan
• BKE40390 (walH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA,  downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC
• BKK40390 (walH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA,  downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC

References

Reviews

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Original Publications

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