epeP

168

membrane protease, processing of modified EpeX

Locus: BSU_40160

Molecular weight: 29.72 kDa

Isoelectric point: 9.64

Protein length: 252 aa

Gene length: 759 bp

Function: control of LiaR-LiaS activity

Product: membrane protease

Essential: no

Synonyms: yydH

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 4,125,742 → 4,126,500

The protein

Catalyzed reaction/ biological activity

• processing of modified EpeX, results in mature peptide (residues 33-49) PubMed

Protein family

• peptidase M50B family (according to UniProt)

Structure

• 3B4R (PDB) (aa 92 ... 224, zinc metalloprotease from Methanocaldococcus jannaschii, 24% identity) PubMed
• Q45594 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: epeX-epeE-epeP-epeA-epeB

  Description: PubMed

  Regulation

  • repressed during logarithmic growth (AbrB) PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • Rok: repression, PubMed, in rok regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B809 (yydH::erm), available at the NBRP B. subtilis, Japan
• BKE40160 (ΔepeP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCATACTTTAAAATTTTC,  downstream forward: _UP4_TAGAGTAAAAGATCTCCAAA
• BKK40160 (ΔepeP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCATACTTTAAAATTTTC,  downstream forward: _UP4_TAGAGTAAAAGATCTCCAAA

References

Reviews

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Original Publications

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