AsnH

• Description: asparagine synthase (glutamine-hydrolysing)
  
  

Gene name	asnH
Synonyms	yxaN
Essential	no
Product	asparagine synthase (glutamine-hydrolysing))
Function	biosynthesis of asparagine
Gene expression levels in SubtiExpress: asnH
Metabolic function and regulation of this protein in SubtiPathways: asnH
MW, pI	85 kDa, 5.528
Gene length, protein length	2241 bp, 747 aa
Immediate neighbours	yxnB, yxaM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AbrB regulon, CodY regulon

The gene

Basic information

• Locus tag: BSU39920

Database entries

• BsubCyc: BSU39920

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H₂O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)

• Protein family: asparagine synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU39920

• Structure:

• UniProt: P42113

• KEGG entry: [3]

• E.C. number: 6.3.5.4

Expression and regulation

• Operon: yxbB-yxbA-yxnB-asnH-yxaM PubMed

• Expression browser: asnH PubMed

• Sigma factor: sigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: