bglP

bglP
168

beta-glucoside permease of the phosphotransferase system, EIIBCA of the PTS, Trigger enzyme, control of LicT activity

Locus
BSU_39270
Molecular weight
64.52 kDa
Isoelectric point
6.81
Protein length
Gene length
Function
beta-glucoside uptake and phosphorylation, control of LicT activity
Product
beta-glucoside permease of the phosphotransferase system
Essential
no
E.C.
2.7.1.69
Synonyms
bglP, sytA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2190 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,033,778  4,035,607
The protein
Protein family
PTS permease, sucrose family PubMed
PTS EIIA domain type-1 (aa 480-584) (according to UniProt)
PTS EIIB domain type-1 (aa 1-86) (according to UniProt)
PTS EIIC domain type-1 (aa 103-459) (according to UniProt)
Structure
Paralogous protein(s)
cell membrane PubMed
Expression and Regulation
Operons
Description
Regulation
induced by salicin (LicT) PubMed
Regulatory mechanism
CcpA: repression, (CcpA binding site overlaps -35 region) PubMed, in ccpA regulon
LicT: anti-termination, via LicT-dependent RNA switch, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HprK, in licT regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An ncRNA is predicted for 'BglP' PubMed
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bglPyxiE

2025-04-15 07:53:20

TPed

109

3430E959FA22F9ED02C53C45C680D768C53B97DA

8A72165F04B8ECFE717CF8873DBC92B33D203CC9

Biological materials
Mutant
GP475 (erm), available in Jörg Stülke's lab PubMed
BKE39270 (bglP::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTGTATACCTCCTTTTT,  downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
BKK39270 (bglP::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTGTATACCTCCTTTTT,  downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
Expression vectors
pGP1290 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Jörg Stülke's lab,
pGP1300 (expression of ''bglP in B. subtilis'', in pBQ200), available in Jörg Stülke's lab
LacZ fusion
pGP600 (in pAC6), available in Jörg Stülke's lab
GFP fusion
GP1266, bglP-cfp, available in Jörg Stülke's lab PubMed
References
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35E6C81BE481C3E57CF47B781580368C3AC15D83

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Time of last update: 2025-04-30 07:23:22

Author of last update: Christoph.elfmann