yxiM

168

similar to rhamnogalacturonan acetylesterase

Locus: BSU_39120

Molecular weight: 41.68 kDa

Isoelectric point: 6.62

Protein length: 382 aa

Gene length: 1149 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yxiM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3401 (Galperin et al., 2021)

Gene

Coordinates: 4,017,508 → 4,018,656

The protein

Protein family

• 'GDSL' lipolytic enzyme family (according to UniProt)

Structure

• 2O14 (PDB)
• P42304 (AlphaFold)

Paralogous protein(s)

• YesY, YesT

Expression and Regulation

Operons

• Genes: yxiM-deaD

  Description: PubMed

  Regulation

  • expression declines in the stationary phase PubMed

Biological materials

Mutant

• MGNA-B719 (yxiM::erm), available at the NBRP B. subtilis, Japan
• GP1116 (spc), available in Stülke lab
• BKE39120 (ΔyxiM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT,  downstream forward: _UP4_TAATACATCATTTTTCGACA
• BKK39120 (ΔyxiM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT,  downstream forward: _UP4_TAATACATCATTTTTCGACA