nupG

168

purine nucleoside transporter

Locus: BSU_39020

Molecular weight: 43.56 kDa

Isoelectric point: 7.76

Protein length: 397 aa

Gene length: 1194 bp

Function: purine uptake

Product: purine nucleoside transporter

Essential: no

Synonyms: nupG, yxjA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1972 (Galperin et al., 2021)

Gene

Coordinates: 4,005,752 → 4,006,945

Phenotypes of a mutant

• a ''nupN nupG'' double mutant is unable to utilize externally provided guanosine PubMed

The protein

Protein family

• concentrative nucleoside transporter (CNT) (TC 2.A.41) family (with NupC and YutK, according to UniProt)

Structure

• 3TIJ (PDB) (from Vibrio cholerae, 25% identity) PubMed
• P42312 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: nupG

  Regulation

  • expression activated by glucose (4.3 fold) PubMed
  • the G-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • G-box: RNA switch, PubMed, in G-box

Biological materials

Mutant

• MGNA-B725 (yxjA::erm), available at the NBRP B. subtilis, Japan
• CS219 (''nupG::aphA3'', available in Colin Harwood's and Jörg Stülke's labs) PubMed
• BKE39020 (ΔnupG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGCAACTCCTATGA,  downstream forward: _UP4_TAGAAACATTGAGCCATATC
• BKK39020 (ΔnupG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGCAACTCCTATGA,  downstream forward: _UP4_TAGAAACATTGAGCCATATC

References

Loading