scoA

168

succinyl CoA:3-oxoacid CoA-transferase (subunit A)

Locus: BSU_38990

Molecular weight: 25.25 kDa

Isoelectric point: 6.61

Protein length: 238 aa

Gene length: 717 bp

Function: 3-hydroxybutyrate utilization

Product: succinyl CoA:3-oxoacid CoA-transferase (subunit A)

Essential: no

E.C.: 2.8.3.5

Synonyms: scoA, yxjD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1788 (Galperin et al., 2021)

Gene

Coordinates: 4,001,976  4,002,692

The protein

Catalyzed reaction/ biological activity

• 3-oxo acid + succinyl-CoA --> 3-oxoacyl-CoA + succinate (according to UniProt)

Protein family

• 3-oxoacid CoA-transferase subunit A family (with YodS, according to UniProt)

Structure

• 3CDK (PDB) (the ScoA-ScoB complex)
• P42315 (AlphaFold)

Paralogous protein(s)

• YodS

Expression and Regulation

Operons

• Genes: hbuT-scoA-scoB-yxjF

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-B728 (yxjD::erm), available at the NBRP B. subtilis, Japan
• BKE38990 (scoA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATGCCCCTTTCGT,  downstream forward: _UP4_GCATCGGGAAAGGGTGAGGC
• BKK38990 (scoA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATGCCCCTTTCGT,  downstream forward: _UP4_GCATCGGGAAAGGGTGAGGC

References

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