qoxD

168

cytochrome aa3 quinol oxidase (subunit IV)

Locus: BSU_38140

Molecular weight: 13.55 kDa

Isoelectric point: 5.94

Protein length: 124 aa

Gene length: 375 bp

Function: respiration

Product: cytochrome aa3 quinol oxidase (subunit IV)

Essential: no

E.C.: 1.10.3.-

Synonyms: qoxD, ipa-40d

Genomic Context

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List of homologs in different organisms, belongs to COG3125 (Galperin et al., 2021)

Gene

Coordinates: 3,914,315  3,914,689

The protein

Catalyzed reaction/ biological activity

• 2 quinol + O₂ --> 2 quinone + 2 H₂O (according to UniProt)

Protein family

• cytochrome c oxidase bacterial subunit 4 family (with CtaF, according to UniProt)

Structure

• P34959 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Additional information

• the QoxA-QoxB-QoxC-QoxD cytochrome aa₃ oxidase complex is sensitive to increased intracellular Mn²⁺ concentrations, this results in the generation of toxic reactive radicals PubMed

Expression and Regulation

Operons

• Genes: qoxA-qoxB-qoxC-qoxD

  Description: PubMed

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • CitB: translation control, [pubmed|], in citB regulon

  Additional information

  • the presence of an iron-responsive element bound by CitB between ''feuA and feuB'' suggests iron-dependent regulation by CitB PubMed

Biological materials

Mutant

• BKE38140 (qoxD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTGTTCAGCAGATTTGTTTG,  downstream forward: _UP4_TAATACAAAAAACCCTCTTC
• BKK38140 (qoxD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTGTTCAGCAGATTTGTTTG,  downstream forward: _UP4_TAATACAAAAAACCCTCTTC

References

Reviews

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Original Publications

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