gerQ

168

spore coat protein, necessary for the proper localization of CwlJ

Locus: BSU_37920

Molecular weight: 20.13 kDa

Isoelectric point: 7.01

Protein length: 181 aa

Gene length: 546 bp

Function: spore Germination

Product: spore coat protein

Essential: no

Synonyms: gerQ, ywdL, ipa-62r

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5889 (Galperin et al., 2021)

Gene

Coordinates: 3,893,441  3,893,986

The protein

Structure

• P39620 (AlphaFold)

Modification

• cross-linked by YabG and Tgl at higher temperatures that induce germination PubMed

Localization

• inner spore coat  PubMed

Expression and Regulation

Operons

• Genes: gerQ

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-B671 (ywdL::erm), available at the NBRP B. subtilis, Japan
• 1G18 ( gerQ::spec), PubMed, available at BGSC
• BKE37920 (gerQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCGTTCCTCCTTTTTA,  downstream forward: _UP4_TAAGAAAAAAGCCAATCACT
• BKK37920 (gerQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCGTTCCTCCTTTTTA,  downstream forward: _UP4_TAAGAAAAAAGCCAATCACT

References

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