rsfA

168

probable regulator of transcription of SigF-dependent genes

Locus: BSU_37620

Molecular weight: 29.58 kDa

Isoelectric point: 5.13

Protein length: 258 aa

Gene length: 777 bp

Function: control of expression of SigF-dependent genes

Product: regulator of SigF activity

Essential: no

Synonyms: rsfA, ywfN, ipa-92r

Genomic Context

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List of homologs in different organisms, belongs to COG5850 (Galperin et al., 2021)

Gene

Coordinates: 3,861,437  3,862,213

Phenotypes of a mutant

• inactivation of ''rsfA'' reduces sporulation efficiency to 11.6% that of wild type cells PubMed

The protein

Domains

• Myb-like domain (aa 1-57) (according to UniProt)

Structure

• P39650 (AlphaFold)

Localization

• forespore (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rsfA

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigF) PubMed

  Regulatory mechanism

  • RsfA: repression, in rsfA regulon

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon
  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-A592 (ywfN::erm), available at the NBRP B. subtilis, Japan
• 1S132 ( rsfA::tet), PubMed, available at BGSC
• BKE37620 (rsfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATCTCAACTCCCATT,  downstream forward: _UP4_TGACATAGAAAAATCCCAAA
• BKK37620 (rsfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATCTCAACTCCCATT,  downstream forward: _UP4_TGACATAGAAAAATCCCAAA

References

Reviews

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Original Publications

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