ywfO

168

putative metal-dependent phosphohydrolase

Locus: BSU_37600

Molecular weight: 50.81 kDa

Isoelectric point: 5.84

Protein length: 433 aa

Gene length: 1302 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ywfO, ipa-93d

Genomic Context

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List of homologs in different organisms, belongs to COG1078 (Galperin et al., 2021)

Gene

Coordinates: 3,859,535  3,860,836

The protein

Domains

• HD domain (aa 61-178) (according to UniProt)

Structure

• 2O6I (PDB) (from Enterococcus faecalis, 50% identity) PubMed
• P39651 (AlphaFold)

Expression and Regulation

Operons

• Genes: ywzC-ywfO-ywgA

  Description: PubMed

Biological materials

Mutant

• MGNA-A585 (ywfO::erm), available at the NBRP B. subtilis, Japan
• BKE37600 (ywfO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTCATTCCCTCTTTTT,  downstream forward: _UP4_TAGAAATTGGAAAGAAGGAA
• BKK37600 (ywfO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTCATTCCCTCTTTTT,  downstream forward: _UP4_TAGAAATTGGAAAGAAGGAA

References

Research papers

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