speB

168

N¹-aminopropylagmatinase

Locus: BSU_37490

Molecular weight: 32.26 kDa

Isoelectric point: 4.77

Protein length: 290 aa

Gene length: 873 bp

Function: spermidine, polyamine biosynthesis

Product: N¹-aminopropylagmatinase

Essential: no

E.C.: 3.5.3.11

Synonyms: speB, ywhG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0010 (Galperin et al., 2021)

Gene

Coordinates: 3,847,853 → 3,848,725

The protein

Catalyzed reaction/ biological activity

• N¹-aminopropylagmatine + H₂O --> spermidine + urea bioRxiv

Protein family

• arginase family (with RocF and HutG, according to UniProt)

Structure

• 3LHL (PDB) (from Clostridium difficile, 41% identity)
• P70999 (AlphaFold)

Expression and Regulation

Operons

• Genes: speE-speB

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A522 (ywhG::erm), available at the NBRP B. subtilis, Japan
• BKE37490 (ΔspeB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATAATACCCTCGCTT,  downstream forward: _UP4_TAAGTAAACATCCAGACGAT
• BKK37490 (ΔspeB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATAATACCCTCGCTT,  downstream forward: _UP4_TAAGTAAACATCCAGACGAT

References

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