albA

168

radical S-adenosylmethionine enzyme, antilisterial bacteriocin (subtilosin) production

Locus: BSU_37370

Molecular weight: 51.35 kDa

Isoelectric point: 6.2

Protein length: 448 aa

Gene length: 1347 bp

Function: antilisterial bacteriocin (subtilosin) production

Product: radical S-adenosylmethionine enzyme

Essential: no

Synonyms: albA, ywiA

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Metabolism, Additional metabolic pathways, Miscellaneous metabolic pathways, Biosynthesis of antibacterial compounds

Lifestyles, Coping with stress, Biosynthesis of antibacterial compounds

List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)

Gene

Coordinates: 3,836,323 → 3,837,669

The protein

Catalyzed reaction/ biological activity

catalyzes thioether bond formation in subtilosin A PubMed

Cofactors

contains two Fe-S clusters PubMed

Structure

6EFN (PDB) (SkfB,corresponds to aa 109 ... 382, 32% identity) PubMed
P71011 (AlphaFold)

Expression and Regulation

Operons

Genes: sboA-sboX-albA-albB-albC-albD-albE-albF-albG

Description: PubMed

Regulation

expressed under anaerobic conditions (ResD) PubMed

Expression

expression is heterogeneous PubMed

Regulatory mechanism

Rok: repression, PubMed, in rok regulon
ResD: activation, PubMed, in resD regulon
AbrB: repression, PubMed, in abrB regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-B263 (ywiA::erm), available at the NBRP B. subtilis, Japan
BKE37370 (ΔalbA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAATACCATCCCCTATAC,  downstream forward: _UP4_CAGCTTATTTAGGAGGGAAA
BKK37370 (ΔalbA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAATACCATCCCCTATAC,  downstream forward: _UP4_CAGCTTATTTAGGAGGGAAA

References

Loading