ywkB

168

unknown

Locus: BSU_37040

Molecular weight: 33.49 kDa

Isoelectric point: 7.11

Protein length: 319 aa

Gene length: 960 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: ywkB

Genomic Context

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List of homologs in different organisms, belongs to COG0679 (Galperin et al., 2021)

Gene

Coordinates: 3,799,377  3,800,336

The protein

Protein family

• auxin efflux carrier (TC 2.A.69) family (single member, according to UniProt)

Structure

• P45869 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: maeA-ywkB

  Description: PubMed

  Regulation

  • expressed in the presence of malate (MalR) PubMed

  Regulatory mechanism

  • MalR: activation, PubMed, in malR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-B656 (ywkB::erm), available at the NBRP B. subtilis, Japan
• BKE37040 (ywkB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAATATTCTCCTCCAAAT,  downstream forward: _UP4_TAACAAAAAAAGCTCCCTTT
• BKK37040 (ywkB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAATATTCTCCTCCAAAT,  downstream forward: _UP4_TAACAAAAAAAGCTCCCTTT

References

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