ywoC

168

unknown

Locus: BSU_36490

Molecular weight: 20.97 kDa

Isoelectric point: 4.62

Protein length: 189 aa

Gene length: 570 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ywoC

Genomic Context

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List of homologs in different organisms, belongs to COG1335 (Galperin et al., 2021)

Gene

Coordinates: 3,755,291 → 3,755,860

The protein

Protein family

• Isochorismatase family (according to UniProt)

Structure

• 1J2R (PDB) (from E. coli, 46% identity)
• P94573 (AlphaFold)

Expression and Regulation

Operons

• Genes: ywoB-ywoC-ywoD

  Description: PubMed

  Regulation

  • induced by ramoplanin (YtrA) PubMed

  Regulatory mechanism

  • YtrA: repression, PubMed, in ytrA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A932 (ywoC::erm), available at the NBRP B. subtilis, Japan
• BKE36490 (ΔywoC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGTCTCCTCCTGATAC,  downstream forward: _UP4_GAGTTTCTGGAACAAGTGAA
• BKK36490 (ΔywoC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGTCTCCTCCTGATAC,  downstream forward: _UP4_GAGTTTCTGGAACAAGTGAA