mscL

168

large conductance mechanosensitive channel protein, prevents selective release of cytoplasmic proteins in a hypotonic environment

Locus: BSU_36360

Molecular weight: 14.14 kDa

Isoelectric point: 4.65

Protein length: 130 aa

Gene length: 393 bp

Function: resistance to osmotic downshock, glycine betaine export

Product: large conductance mechanosensitive channel protein

Essential: no

Synonyms: mscL, ywpC

Genomic Context

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List of homologs in different organisms, belongs to COG1970 (Galperin et al., 2021)

Gene

Coordinates: 3,743,267  3,743,659

Phenotypes of a mutant

• sensitive to osmotic downshock (> 0.5 M) during logarithmic growth PubMed

The protein

Protein family

• mscL family (single member, according to UniProt)

Structure

• 3HZQ (PDB) (MscL of Staphylococcus aureus) PubMed
• P94585 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: ywpB-mscL

  Description: PubMed

  Regulation

  • expressed in logarithmic phase PubMed

Biological materials

Mutant

• MGNA-A562 (ywpC::erm), available at the NBRP B. subtilis, Japan
• 1A957 ( mscL::spec), PubMed, available at BGSC
• BKE36360 (mscL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAATCACCTGCTTTTC,  downstream forward: _UP4_TAAAAAAGATGCCGTTAGAA
• BKK36360 (mscL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAATCACCTGCTTTTC,  downstream forward: _UP4_TAAAAAAGATGCCGTTAGAA

References

Reviews

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Original Publications

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Labs working on this gene/protein

• Jan Maarten van Dijl, Groningen, Netherlands
• Erhard Bremer, University of Marburg, Germany homepage