capC

168

part of the CapB-CapC glutamic acid ligase, capsular polyglutamate biosynthesis

Locus: BSU_35890

Molecular weight: 16.16 kDa

Isoelectric point: 9.67

Protein length: 149 aa

Gene length: 450 bp

Function: capsule synthesis

Product: unknown

Essential: no

Synonyms: capC, ywtA, pgsC

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,698,982 → 3,699,431

Phenotypes of a mutant

• no synthesis of the poly-gamma-glutamate capsule PubMed

The protein

Structure

• P96737 (AlphaFold)

Expression and Regulation

Operons

• Genes: capB-capC-capA-capE

  Description: PubMed

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in degU regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • expression of the operon is increased in 'motA' or 'motB' mutants due to increased DegU phosphorylation PubMed

Biological materials

Mutant

• MGNA-A076 (ywtA::erm), available at the NBRP B. subtilis, Japan
• BKE35890 (ΔcapC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCGAACATGTCTGCATTTC,  downstream forward: _UP4_ATTTAATGTAAGGTGTGTCA
• BKK35890 (ΔcapC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCGAACATGTCTGCATTTC,  downstream forward: _UP4_ATTTAATGTAAGGTGTGTCA

References

Reviews

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Original Publications

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