gerBB

168

nutrient receptor, germination response to the combination of glucose, fructose, and KCl

Locus: BSU_35810

Molecular weight: 41.55 kDa

Isoelectric point: 9.89

Protein length: 368 aa

Gene length: 1107 bp

Function: germination

Product: part of the putative nutrient-gated GerBA-GerBB-GerBC ion channel

Essential: no

Synonyms: gerBB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5902 (Galperin et al., 2021)

Gene

Coordinates: 3,690,269 → 3,691,375

The protein

Catalyzed reaction/ biological activity

• the GerBA-GerBB-GerBC ion channel likely releases ions from the spore core in response to asparagine, glucose, fructose and K⁺ to trigger Germination, this requires the gerK locus PubMed

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)
• Spore germination protein (SGP) (TC 2.A.3.9) family (according to UniProt)

Structure

• P39570 (AlphaFold)

Paralogous protein(s)

• GerAB, YndE, GerKB

Localization

• spore inner membrane PubMed

Expression and Regulation

Operons

• Genes: gerBA-gerBB-gerBC

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

  Additional information

  • 700 molecules are present per spore PubMed

Biological materials

Mutant

• BKE35810 (ΔgerBB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCTCTGATTTCCTCATAG,  downstream forward: _UP4_AAAATAGGGAGGGGGCAGCT
• BKK35810 (ΔgerBB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCTCTGATTTCCTCATAG,  downstream forward: _UP4_AAAATAGGGAGGGGGCAGCT

References

Reviews

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Original Publications

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