mdxR

168

transcriptional activator (LacI family), regulator of starch and maltodextrin utilization

Locus: BSU_34630

Molecular weight: 35.28 kDa

Isoelectric point: 7.09

Protein length: 316 aa

Gene length: 951 bp

Function: regulation of starch and maltodextrin utilization

Product: transcriptional activator (LacI family)

Essential: no

Synonyms: yvdE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1609 (Galperin et al., 2021)

Gene

Coordinates: 3,557,784 → 3,558,734

The protein

Catalyzed reaction/ biological activity

• binds to the promoter region of the mdxR-yvdF-mdxE-mdxF-mdxG-yvdJ-yvdK-malL-pgcM operon and activates transcription PubMed

Protein family

• LacI family

Domains

• HTH lacI-type domain (aa 1-56) (according to UniProt)

Structure

• 5YSZ (PDB) (from Thermobifida fusca, 30% identity) PubMed
• O06987 (AlphaFold)

Expression and Regulation

Operons

• Genes: mdxR-yvdF-mdxE-mdxF-mdxG-yvdJ-yvdK-malL-pgcM

  Description: PubMed

  Regulation

  • induced in the presence of starch, β-cyclomaltodextrin, or maltose PubMed

  Regulatory mechanism

  • MdxR: activation, PubMed, in mdxR regulon

Biological materials

Mutant

• BKE34630 (ΔmdxR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCATCCCCTCCTTAT,  downstream forward: _UP4_TAAGCGCCTTATTCTGTTAT
• BKK34630 (ΔmdxR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCATCCCCTCCTTAT,  downstream forward: _UP4_TAAGCGCCTTATTCTGTTAT

References

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