malL

168

alpha-glucosidase

Locus: BSU_34560

Molecular weight: 65.91 kDa

Isoelectric point: 4.98

Protein length: 561 aa

Gene length: 1686 bp

Function: starch and maltodextrin utilization

Product: alpha-glucosidase

Essential: no

E.C.: 3.2.1.10

Synonyms: malL, yvdL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)

Gene

Coordinates: 3,547,550  3,549,235

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose (according to UniProt)

Protein family

• glycosyl hydrolase 13 family (according to UniProt)

Structure

• 1UOK (PDB) (from Bacillus cereus, 57% identity, 74% similarity)\tPubMed
• O06994 (AlphaFold)

Paralogous protein(s)

• YcdG, YugT, TreA

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: mdxR-yvdF-mdxE-mdxF-mdxG-yvdJ-yvdK-malL-pgcM

  Description: PubMed

  Regulation

  • induced in the presence of starch, β-cyclomaltodextrin, or maltose PubMed

  Regulatory mechanism

  • MdxR: activation, PubMed, in mdxR regulon

Biological materials

Mutant

• BKE34560 (malL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATAAACGACAGCTTCTTTCC,  downstream forward: _UP4_GAAGCTGTGATGGGCATTAG
• BKK34560 (malL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATAAACGACAGCTTCTTTCC,  downstream forward: _UP4_GAAGCTGTGATGGGCATTAG

References

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