levB

168

endolevanase

Locus: BSU_34460

Molecular weight: 58.81 kDa

Isoelectric point: 4.99

Protein length: 516 aa

Gene length: 1551 bp

Function: levan degradation

Product: endolevanase

Essential: no

E.C.: 3.2.1.64

Synonyms: levB, yveB

Genomic Context

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List of homologs in different organisms, belongs to COG1621 (Galperin et al., 2021)

Gene

Coordinates: 3,537,507  3,539,057

Phenotypes of a mutant

• defective in tomato root colonization in the presence of sucrose PubMed

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain (according to UniProt)

Protein family

• glycosyl hydrolase 32 family (with SacC and SacA, according to UniProt)

Structure

• 4FFF (PDB) (from Arthrobacter ureafaciens, 42% identity) PubMed
• O07003 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: sacB-levB

  Description: PubMed

  Regulation

  • the RNA switch RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in degU regulon
  • SacY: antitermination, /antitermination via binding to a RNA switch, in sacY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B614 (yveB::erm), available at the NBRP B. subtilis, Japan
• BKE34460 (levB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGGGATTCACCTTTAT,  downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG
• BKK34460 (levB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGGGATTCACCTTTAT,  downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG

References

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