epsD

168

UDP-GlcNAc-dependent N-acetyl glucosamine transferase , extracellular polysaccharide synthesis

Locus: BSU_34340

Molecular weight: 43.41 kDa

Isoelectric point: 8.73

Protein length: 381 aa

Gene length: 1146 bp

Function: biofilm formation

Product: UDP-GlcNAc-dependent N-acetyl glucosamine transferase

Essential: no

Synonyms: epsD, yveN

Genomic Context

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List of homologs in different organisms, belongs to COG0438 (Galperin et al., 2021)

Gene

Coordinates: 3,525,250  3,526,395

Phenotypes of a mutant

• smooth colonies on MsGG medium, no biofilm formation PubMed

The protein

Catalyzed reaction/ biological activity

• undecaprenyl-PP-diNAcBac + N-acetyl-glucosamine --> undecaprenyl-PP-diNAcBac-NAc-glucosamine + UDP PubMed

Protein family

• glycosyltransferase 1 family (according to UniProt)
• Glycosyltransferase 4 subfamily (according to UniProt)

Structure

• 2JJM (PDB) (from B. anthracis, 24% identity) PubMed
• P71053 (AlphaFold)

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • RemA: activation, PubMed, in remA regulon
  • AbrB: repression, PubMed, in abrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed

Biological materials

Mutant

• MGNA-A070 (yveN::erm), available at the NBRP B. subtilis, Japan
• BKE34340 (epsD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCACTCCCCCTAATG,  downstream forward: _UP4_GTATGAACTCAGGACCGAAA
• BKK34340 (epsD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCACTCCCCCTAATG,  downstream forward: _UP4_GTATGAACTCAGGACCGAAA

Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

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Research papers

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The EAR RNA switch

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