ganP

168

galactotriose ABC transporter (permease)

Locus: BSU_34150

Molecular weight: 46.82 kDa

Isoelectric point: 6.09

Protein length: 418 aa

Gene length: 1257 bp

Function: uptake of galactotriose

Product: galactotriose ABC transporter (permease)

Essential: no

Synonyms: ganP, yvfL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)

Gene

Coordinates: 3,505,957  3,507,213

Phenotypes of a mutant

• a ''ganS-ganP-ganQ-ganA-ganB'' mutant is impaired in the utilization of galactan PubMed

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 187-407) (according to UniProt)

Structure

• 2R6G (PDB) (from E. coli, C-terminal domain, aa 165-412, 41% identity) PubMed
• O32261 (AlphaFold)

Paralogous protein(s)

• MdxF

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: ganS-ganP-ganQ-ganA-ganB

  Description: PubMed

  Regulation

  • induced by galactan (binding of galactobiose to GanR) PubMed
  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • GanR: repression, PubMed, in ganR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A485 (yvfL::erm), available at the NBRP B. subtilis, Japan
• BKE34150 (ganP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCGTTTTCACCTCTCTTA,  downstream forward: _UP4_TCTTTTAAAGAGGAGGCATA
• BKK34150 (ganP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCGTTTTCACCTCTCTTA,  downstream forward: _UP4_TCTTTTAAAGAGGAGGCATA

References

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