ganB

168

endo-1,4-beta-galactosidase

Locus: BSU_34120

Molecular weight: 47.12 kDa

Isoelectric point: 8.24

Protein length: 429 aa

Gene length: 1290 bp

Function: galactan utilization

Product: endo-1,4-beta-galactosidase

Essential: no

E.C.: 3.2.1.89

Synonyms: ganB, yvfO

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3867 (Galperin et al., 2021)

Gene

Coordinates: 3,501,651  3,502,940

Phenotypes of a mutant

• a ''ganS-ganP-ganQ-ganA-ganB'' mutant is impaired in the utilization of galactan PubMed

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (14)-beta-D-galactosidic linkages in galactan, generation of galactotriose PubMed

Protein family

• glycosyl hydrolase 53 family (single member, according to UniProt)

Structure

• 2J74 (PDB) (B. licheniformis)
• O07013 (AlphaFold)

Localization

• likely to be extracellular, carries a predicted a signal peptide of 26 amino acid residues at the N-terminus PubMed

Expression and Regulation

Operons

• Genes: ganS-ganP-ganQ-ganA-ganB

  Description: PubMed

  Regulation

  • induced by galactan (binding of galactobiose to GanR) PubMed
  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • GanR: repression, PubMed, in ganR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A487 (yvfO::erm), available at the NBRP B. subtilis, Japan
• BKE34120 (ganB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTTCATCCCCCCAAG,  downstream forward: _UP4_TGAAACAAAAAAATCCATGA
• BKK34120 (ganB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTTCATCCCCCCAAG,  downstream forward: _UP4_TGAAACAAAAAAATCCATGA

References

Loading