smpB

168

tmRNA-binding protein

Locus: BSU_33600

Molecular weight: 17.95 kDa

Isoelectric point: 10.42

Protein length: 156 aa

Gene length: 471 bp

Function: trans-translation

Product: tmRNA-binding protein

Essential: no

Synonyms: smpB, yvaI

Genomic Context

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List of homologs in different organisms, belongs to COG0691 (Galperin et al., 2021)

Gene

Coordinates: 3,451,248 → 3,451,718

Phenotypes of a mutant

• impaired growth at high and low temperatures PubMed
• inactivation of ''smpB'' reduces sporulation efficiency to 23% that of wild type cells; delayed entry into sporulation PubMed
• a brfA smpB double mutant has a severe growth defect PubMed
• a rcqH smpB double mutant has a severe growth defect PubMed

The protein

Protein family

• smpB family (single member, according to UniProt)

Structure

• 1P6V (PDB) (from Aquifex aeolicus, 55% identity, complex with the tRNA domain of SsrA) PubMed
• O32230 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: secG-yvaK-rnr-smpB-ssrA

  Description: PubMed

  Regulation

  • ''yvaK'': induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

• Genes: smpB-ssrA

  Description: PubMed

  Regulation

  • ''yvaK'': SigB PubMed

• Genes: yvaK-rnr-smpB-ssrA

  Description: PubMed

  Regulation

  • ''yvaK'': induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-A494 (yvaI::erm), available at the NBRP B. subtilis, Japan
• BKE33600 (ΔsmpB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCAGAACCTCCTCTCT,  downstream forward: _UP4_TAAAGGCATAGTGCTTGATT
• BKK33600 (ΔsmpB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCAGAACCTCCTCTCT,  downstream forward: _UP4_TAAAGGCATAGTGCTTGATT

Labs working on this gene/protein

• Chet Price, Davis, USA homepage

References

Reviews

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Original Publications

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