helD

168

DNA 3-5 helicase IV, removes and recycles stalled transcription complexes

Locus: BSU_33450

Molecular weight: 89.74 kDa

Isoelectric point: 6.08

Protein length: 774 aa

Gene length: 2325 bp

Function: transcription

Product: ATP-dependent transcription recycling factor

Essential: no

E.C.: 3.6.4.12

Synonyms: helD, yvgS

Genomic Context

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Categories containing this gene/protein

Information processing, RNA synthesis and degradation, Transcription, Transcription elongation/ termination

List of homologs in different organisms, belongs to COG3973 (Galperin et al., 2021)

Gene

Coordinates: 3,434,327  3,436,651

The protein

Catalyzed reaction/ biological activity

removes nucleic acids from the active site of RNA polymerase, and induces a large conformational change in the primary channel of RNA polymerase leading to removal and recycling of the stalled polymerase PubMed

Protein family

helicase family (according to UniProt)

Domains

an N-terminal region (NTR; residues 4–187) PubMed
UvrD-like helicase ATP-binding domain (aa 212-610) consisting of two globular domains (D1a/D1b, residues 188–338/491–603; D2, residues 604–774) PubMed
an elongated helical protrusion in D1 (HelDBumper; residues 339–490) PubMed

Structure

3DMN (PDB) (C-terminal domain of the protein from Lactobacillus plantarum, 39% identity)
O32215 (AlphaFold)

Localization

cytoplasm (according to UniProt)

Expression and Regulation

Operons

Genes: helD

Description: PubMed

Biological materials

Mutant

MGNA-A456 (yvgS::erm), available at the NBRP B. subtilis, Japan
1A899 ( helD::erm), available at BGSC
BKE33450 (helD::erm  trpC2) available at BGSC as well as in Fabian Commichau's and Jörg Stülke's labs, PubMed, upstream reverse: _UP1_CATGAACGGCACCTCCAGAA,  downstream forward: _UP4_TGAAGAGAAGGGAGTCCTTC
BKK33450 (helD::kan  trpC2) available at BGSC and in Jörg Stülke's lab,  PubMed, upstream reverse: _UP1_CATGAACGGCACCTCCAGAA,  downstream forward: _UP4_TGAAGAGAAGGGAGTCCTTC

References

Reviews

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Original Publications

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