yvsH

168

putative lysine transporter

Locus: BSU_33330

Molecular weight: 50.09 kDa

Isoelectric point: 9.74

Protein length: 469 aa

Gene length: 1410 bp

Function: uptake of lysine

Product: putative lysine transporter

Essential: no

Synonyms: yvsH, lysP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0531 (Galperin et al., 2021)

Gene

Coordinates: 3,419,656  3,421,065

The protein

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

Structure

• 3LRB (PDB) (from E. coli, 32% identity) PubMed
• O32204 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: yvsH

  Description: PubMed

  Regulation

  • antitermination is modulated by NusG-dependent transcription pausing just upstream of the L-box PubMed

  Regulatory mechanism

  • L-box: RNA switch, via a riboswitch, in L-box

Biological materials

Mutant

• GP4153 (ΔyvsH::aphA3), available in Jörg Stülke's lab
• MGNA-B067 (yvsH::erm), available at the NBRP B. subtilis, Japan
• BKE33330 (yvsH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCAGTACCTCCATGAT,  downstream forward: _UP4_TAATGAAAAGACACCTGATT
• BKK33330 (yvsH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCAGTACCTCCATGAT,  downstream forward: _UP4_TAATGAAAAGACACCTGATT

References

Loading