yvrO

168

similar to amino acid [wiki|ABC transporter ](ATP-binding protein)

locus: BSU_33270

Molecular weight: 25.33 kDa

pI: 9.7

Protein length: 229 aa

Gene length: 690 bp

function: unknown

product: [wiki|ABC transporter ](ATP-binding protein)

essential: no

synonyms: yvrO

List of homologs in different organisms, belongs to COG1136 (Galperin et al., 2021)

Gene

Coordinates: 3,413,350  3,414,039

The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 2-229) (according to UniProt)

Structure

• [PDB|1L2T] (from Methanocaldococcus jannaschii, 51% identity) [pubmed|12150914]
• [AF|O34979]

Modification

• phosphorylated on Arg-161 [Pubmed|22517742]

Paralogous protein(s)

• [protein|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA], [protein|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ], [protein|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|ytrE], [protein|6882EF93EC82872B25C88104F62D6603D2514890|artR], [protein|8B4267532B9788AEB5EF39E2695C1FDA494C7055|yxdL], [protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE], [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA], [protein|B23CAC2563A36415C482AE2FA695E09228EF5B32|yclH], [protein|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN], [protein|B453986691E1231A1C565D469A2A60EBFF2F6BD3|tcyC], [protein|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO], [protein|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]

[wiki|Localization]

• membrane associated (via [protein|5AEDC5B857537B7D2A2637C0BC5BE78FD09A66B3|yvrN]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|7D334BBF7628D1DA001727CF7BEDB4EFA7FF88BA|yvrP]-[gene|0F8048267EC038D8CF673317D969BA27735473A7|yvrO]-[gene|5AEDC5B857537B7D2A2637C0BC5BE78FD09A66B3|yvrN]

  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

Biological materials

Mutant

• MGNA-B057 (yvrO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1056 NBRP B. subtilis, Japan]
• BKE33270 ([gene|0F8048267EC038D8CF673317D969BA27735473A7|yvrO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATTATTCAGTGTCAGCATT,  downstream forward: _UP4_AGCCATGCTTGAACATATTC
• BKK33270 ([gene|0F8048267EC038D8CF673317D969BA27735473A7|yvrO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATTATTCAGTGTCAGCATT,  downstream forward: _UP4_AGCCATGCTTGAACATATTC

References

• 10092453,11948146,22517742,20817675,12150914