yvrG

168

two-component sensor kinase of the YvrG-YvrHb two-component system

Locus: BSU_33210

Molecular weight: 66.58 kDa

Isoelectric point: 9.52

Protein length: 580 aa

Gene length: 1743 bp

Function: regulation cell surface maintenance

Product: two-component sensor histidine kinase

Essential: no

E.C.: null

Synonyms: yvrG

Genomic Context

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List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)

Gene

Coordinates: 3,406,614  3,408,356

Phenotypes of a mutant

• unusual autolysis and higher susceptibility to cell envelope antibiotics (bacitracin, aztreonam, cefepime, fosfomycin)  PubMed

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of the YvrHb response regulator  PubMed
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• two transmembrane segments
• Histidine kinase domain (aa 363-580) (according to UniProt)

Structure

• O34989 (AlphaFold)

Modification

• autophosphorylation on a His residue

Paralogous protein(s)

• YkoH, KinE, CssS, YclK

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yvrHb-yvrG-yvrE

  Description: PubMed

Biological materials

Mutant

• MGNA-B049 (yvrG::erm), available at the NBRP B. subtilis, Japan
• BKE33210 (yvrG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTCATGACCGCTTGCCTTC,  downstream forward: _UP4_TAAACATCAAGTGGTTTGGA
• BKK33210 (yvrG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTCATGACCGCTTGCCTTC,  downstream forward: _UP4_TAAACATCAAGTGGTTTGGA

References

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